Gas-phase fragmentation characteristics of benzyl-aminated lysyl-containing tryptic peptides
نویسندگان
چکیده
منابع مشابه
Selective collision-induced fragmentation of ortho-hydroxybenzyl-aminated lysyl-containing tryptic peptides.
RATIONALE In protein studies that employ tandem mass spectrometry the manipulation of protonated peptide fragmentation through exclusive dissociation pathways may be preferred in some applications over the comprehensive amide backbone fragmentation that is typically observed. In this study, we characterized the selective cleavage of the side-chain Cζ-Nε bond of peptides with ortho-hydroxybenzyl...
متن کاملSubstituent effects on the gas-phase fragmentation reactions of protonated peptides containing benzylamine-derivatized lysyl residues.
Motivated by the need for chemical strategies designed to tune peptide fragmentation to selective cleavage reactions, benzyl ring substituent influence on the relative formation of carbocation elimination (CCE) products from peptides with benzylamine-derivatized lysyl residues has been examined using collision-induced dissociation (CID) tandem mass spectrometry. Unsubstituted benzylamine-deriva...
متن کاملCis-trans signatures of proline-containing tryptic peptides in the gas phase.
High-resolution ion mobility/time-of-flight techniques were used to measure collision cross sections for 968 tryptic digest peptide ions obtained from digestion of common proteins. Here, we report a mobility signature that aids in identifying proline-containing peptides containing 4-10 residues. Of 129 peptides (< or = 10 residues in length) in the database that contain proline residues, 57% sh...
متن کاملSubstituent effects on the gas-phase fragmentation reactions of sulfonium ion containing peptides.
The multistage mass spectrometric (MS/MS and MS3) gas-phase fragmentation reactions of methionine side-chain sulfonium ion containing peptides formed by reaction with a series of para-substituted phenacyl bromide (XBr where X=CH2COC6H4R, and R=--COOH, --COOCH3, --H, --CH3 and --CH2CH3) alkylating reagents have been examined in a linear quadrupole ion trap mass spectrometer. MS/MS of the singly ...
متن کاملObservation of conserved solution-phase secondary structure in gas-phase tryptic peptides.
Results from ion mobility studies of tryptic peptides suggest that, in some cases, the gas-phase structures can be related to the solution-phase structure of the parent protein. The interpretation of ion mobility measurements is supported by results from molecular modeling and H/D exchange experiments on the same peptides. This study clearly illustrates the utility of IM-MS for screening comple...
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ژورنال
عنوان ژورنال: Journal of the American Society for Mass Spectrometry
سال: 2010
ISSN: 1044-0305,1879-1123
DOI: 10.1016/j.jasms.2010.04.006